Electrochemical Biosensors for the Determination of the Antioxidant Capacity
نویسنده
چکیده
An amperometric cytochrome c-based electrode was developed and applied to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently immobilizing both cyt c and XOD on a mercaptoundecanoic acid/mercaptoundecanol mixed SAMmodified screen-printed gold electrode. The concentration which induces a 50% inhibition of the superoxide level (IC50) has been determined for different substances, for instance pure substances such as ascorbic acid or Trolox, and natural sources of antioxidants, particularly orange juices and garlic extracts.
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